Features to Help Make Calls in the Mixture Interpretation Tool

There are several additional features in the Mixture Interpretation Tool to aide you in making calls.  This guide goes through each of the features and explains how to use them.

Highest to Lowest # option 

By clicking on the box next to Highest to Lowest #, the Loci will be arranged for selection from the locus with the most alleles to the ones with the least.  Note:   Changes made to the alleles while making your interpretation, like deleting an allele or adding stutter, can change the order of the Loci when using the Highest to Lowest # option.

View call report

To assist with your interpretation you can choose to visualize the proportions of your calls in a graphical format by clicking on the “View call report” button.  A report will open that will show each Locus for each profile and as you make your calls, the alleles will auto populate the report.  A graph showing the proportion of each profile by locus is generated as shown in image shown below.  You can customize how the report appears by clicking the arrow next to “View call report” button.  It will bring up a drop down list as shown below. 

NOTE: The report only shows the Loci that you have completed using the Mixture Interpretation Window.  The graph only shows the proportions for the loci that have completed combinations called.  

The Call Report includes additional worksheets to document each of your interpretation parameters and results locus-by-locus.  If you click on the Mix_Interp Worksheet tab at the bottom of the report, you will see a Mixture Interpretation Detail Report.  NOTE: The report only shows the Loci that you have went to using the Mixture Interpretation Window.  

These reports can be printed and/or saved using the print and export features explained in Chapter 2.

You can add a comment(s) to the call report.  Click on “Add Comment” button.  A drop down list will appear to give you a choice to add comments to the Calls page or the Mixt Interp page or you can remove comments that you have already added.  If you select to add comment to either page, the “Add a comment” window will open.  Type the comment you would like to appear on the page.  Click “Ok”. This will add a comment to the specific page in the Call report.    

Proportion Average and Dilution Ratio Shown

As you make each call, the proportion average will be shown on the Mixture Interpretation Window.  It is located next to the Highest to Lowest # feature.  Next to the proportions will be the dilution ratio, if that option is selected in the Mixture Interpretation settings in the ArmedXpert Options window.  

Operations Button

There is an “Operations” button to the right of the selected sample.  This opens a RMP report for this sample or allows you to open an OSIRIS Plot File or Virtual Electropherogram. 

Click on the “Operations” button and a drop down list will open. 

To open the RMP report, click on RMP.   The Locus you currently are on will be shown.  You can choose between Modified RMP, Forced mRMP, and Restricted RMP.  If you would like to have the report shown all Loci with Restricted RMP, click on the box next to “Apply Restricted Globally.” If you do not, all Loci will have the Modified RMP applied.  Make sure the Contributor number is correct. Finally, click the “Open Frequency Report” button.  A mixture frequency report will then open to aide you with making your calls.

To open an OSIRIS Plot File, choose OSIRIS Plot File from the list.  You can change from a full e-gram, single plot, or multiple plots by choosing each option in the drop down list once the OSIRIS Plot File is open.  

To open a Virtual Electropherogram, choose Virtual Electropherogram from the list.  You can change from a full e-gram, single plot, or multiple plots by choosing each option in the drop down list once the virtual Electropherogram is open.