How to Generate a Peak Height Ratio Validation Report

The PHR Report shows all the peak height ratios (PHR) as well as the average PHR for selected table(s) or sample(s). This is useful to determine thresholds.  This guide explains how to create the PHR Report.

Create Dilution Tables

Import your dilution sample table into ArmedXpert. After importing table, click on Combine Sources under the Data tab. The Combine Sources window will open. Type the dilution you would like to create a table for in the selection filter box. Click the > button to move all highlighted samples to the right hand box. Title of the table you are creating. Click on the “Combine” button. Your new combined sample table will open.

In this displayed example, 1.0ng was entered in the selection filter box. All samples containing 1.0ng were highlighted in the box on the left hand side. Move your selections to the right hand box by using the > button.  1.0 dilution was added as the Title.  Repeat to create different dilution tables that you would like to see on the PHR report. Once finished, click the X on the upper right hand of the window to close the Combine Sources Window.

Remove the selected samples from the right hand side using the < button before creating each new dilution (sample) table.

Run PHR Report

Click on PHR Report under the Reporting Tab and the PHR Report Window will open. Click on “Add a table” button.

The Select a table Window will open. You can either select an entire table or expand the table and select individual sample(s). Click the > button to move the selected table or sample to the right side.

Once all tables or samples have been selected and moved to the right side, click on the “Ok” button.

The PHR Report will open back up. Your chosen selections will appear.

If you would like to change any of the table‟s description names before you run the report, double click on the table from the list shown. An Enter a description Window will open. Type in the new description and click on the “Ok” button.

Once finished, click the “Ok” button on the PHR Report window to run the PHR report.

PHR Report for All Loci

The PHR report shows the PHR at each Locus for each sample chosen.  Any Locus that does not contain data because there was only one allele at that Locus can be hidden from the report to make reading easier.

To hide a Locus, click on the Locus title (multiple Loci can be selected by holding down the Ctrl key and clicking on each Locus).  Then hit the F5 button.  All selected Loci will be hidden from the report.

To redisplay the hidden Loci, hit the F6 button.

PHR Report for Only Selected Loci

On the left hand side of the report is the PHR at each Locus.  The average PHR can be seen at the end of each table, as well as the standard deviation, the minimum PHR, the maximum PHR, and the standard error.  A graph appears to the right of the Loci that displays the average PHR for each Locus by Dilution table.